PCR steps listed in the material

PCR steps listed in the material

• A. Nucleotide extraction; PCR amplification; pathogen detection
• B. Protein synthesis; cell culture
• C. RNA transcription; translation
• D. Antibody testing

Answer: (A)

PCR works by a simple workflow: first you obtain the target genetic material from the sample by extracting nucleic acids, then you amplify the specific DNA (or cDNA in the case of RNA targets) so there are enough copies to detect, and finally you analyze or detect the amplified product to determine if the pathogen’s genetic sequence is present. That sequence—extraction, amplification, detection—captures how a PCR test is performed. The other options describe processes like protein synthesis or cell culture, general gene expression, or antibody-based tests, which are not part of the PCR workflow.

PCR works by a simple workflow: first you obtain the target genetic material from the sample by extracting nucleic acids, then you amplify the specific DNA (or cDNA in the case of RNA targets) so there are enough copies to detect, and finally you analyze or detect the amplified product to determine if the pathogen’s genetic sequence is present. That sequence—extraction, amplification, detection—captures how a PCR test is performed. The other options describe processes like protein synthesis or cell culture, general gene expression, or antibody-based tests, which are not part of the PCR workflow.